功能矫形前伸大鼠下颌后髁突胰岛素样生长因子Ⅰ表达变化的研究

Induction of Insulinlike Growth Factor Ⅰ Expression to Mandibular Advancement in Growing Rat Condylar Cartilage

采用免疫组织化学技术结合数字图像分析，对功能矫治器前伸大鼠下颌后，髁突软骨中胰岛素样生长因子Ｉ（ＩＧＦ－Ⅰ）表达变化的规律进行研究，以探讨功能矫形治疗的分子机制。结果显示：髁突软骨中滑膜层、纤维层和生发层细胞中ＩＧＦ－Ⅰ阳性最强，在过渡层中较强，成熟层和移行层中较弱，在骨小梁中的成骨细胞也有阳性信号；实验组各层细胞阳性强度高于对照组，并且与功能矫形引起的组织学改变和细胞功能变化一致。

It was studied effects of functional mandibular advancement on IGFⅠ peptide of condylar cartilage of 60 5weekold male rats. Animals were randomly divided into the experimental and control groups,and the mimic functional appliances were used in experimental group. The rats were killed after 1, 3, 7, 14, 21 and 28 days. The results showed that the condylar cartilage of growing rats expressed IGFⅠ the strongest in the germinal layer,medial in the transitional layer,and the least in the matutational layer. IGFⅠ positive cells and their immunoreactive levels increased after 1 week of functional mandibular advancement,and reached the peak level at 2 weeks.The results suggest IGFⅠ in mandibular condyle of rat is related to its growth and differentiation activity,and changes of condylar IGFⅠ after functional protrusion are relevant to the histologic changes and celluar functions which indicate their involvement with active bone growth and remodeling.