卵巢囊腺癌抑癌基因p16检测及其意义

p16 gene expression in ovarian cystadenocarcinoma

目的：进一步探讨新型抑癌基因ｐ１６与人类卵巢癌发生发展的关系。方法：采用Ｓ－Ｐ免疫组化方法对母系ＨＯ－８９１０细胞系、高转移ＨＯ－８９１０ＰＭ、ＮＳＭＯ模型和转移瘤以及６９例卵巢囊腺癌手术标本石蜡组织进行ｐ１６检测。结果：母系ＨＯ－８９１０细胞与高转移ＨＯ－８９１０ＰＭ细胞、ＮＳＭＯ模型及转移瘤均具有ｐ１６阳性表达，表达强度均以转移细胞为弱。６９例卵巢癌石蜡组织检测ｐ１６阳性５１例（占７３．９％），结合ｐ１６表达有无及强弱与病理组织学类型及分级、临床期别、腹水、癌肿播散、淋巴结转移及５年生存情况比较分析，发现随恶性程度、期别的增加，癌肿播散、淋巴结转移的出现，５年生存率的减低，ｐ１６表达明显减少，阳性强度减弱，具有统计学差异（Ｐ＜０．０１）。结论：ｐ１６基因不仅为细胞周期的固有调控者，亦与抑制肿瘤生长的关系密切，其缺失与表达水平的改变与卵巢癌的发生发展有关。

Purpose To study the relation between suppresser gene p16 and human cancer occurrence and development. Methods p16 gene expression in HO8910 cell of mother line, highly metastasizing models and xenohrafts of 8910 PM and NSMO, and paraffin embedded tissues from 69 patients with cystadenocarcinoma was measured by SP immunohistochemical method. Results Positive expression of p16 gene protein was found in HO8910 PM cell, model and xenograft of NSMO. Expression of p16 gene protein in metastatic cells was weaker. Positive expression of p16 gene protein was also found in paraffin embedded tissues of 51 cases with ovarian carcinoma, which accounted for 739% of total 69 cases. The positivity rate of p16 gene protein expression markedly reduced with the increase of pathologic grade and clinical stage, dissemintion, lymphnode metastasis and decrease of 5year survival (P<001). Conclusions p16 gene is not only a controller of cytokerastic cycle, but also a key member of tumorigenic suppresser and its absence and change of expression level are also correlated with ovarian carcinoma genesis and development.