马尾神经根横断后修复方法的实验观察

An experimental observation of repair after transection of cauda equina fibers in cat

目的探讨治疗马尾神经离断的更有效方法。方法作者用缝合法、纤维素胶法修复猫椎管内一侧横断的腰６、腰７神经根，并以对侧自然连接做为对照；对神经轴突行免疫组化特异染色，图像分析。结果马尾神经损伤后不但在吻合口前后有明显的轴突再生，在相应的脊髓和神经节内的神经元均有明显的再生表现；神经根横断前后及取标本前的诱发电位变化提示神经根损伤与再生的功能变化过程；在再生的过程中运动根的再生优于感觉根的再生；感觉根的再生主要受阻于后根与脊髓的移行部位。结论实验结果显示纤维素胶粘合法可以替代传统的缝合法，而且比缝合法有更多的优越性。

Objective To explore the regeneration characteristics after cauda equina transection.Methods Fibrin glue was chosen to repair transected left L 6,7 roots in cat,and right roots in situ of the transected ends were served as the control.The characteristics of regeneration were observed by immunohistocytochemistry and morphometric analysis.Results Regeneration was found not only in site of anastomosis,but also in corresponding spinal segments and DRGs.Sensory and motor evoked potentials(SEP and MEP)performed before and after transection and before taking specimen revealed the course of functional recovery on severed and regenerated nerve roots.The regeneration of moter roots was better than that of sensory roots,and they were mainly blocked at the site of central peripheral transition zone.Conclusiions The method of adhesion by fibrin glue can replace traditional suture,and it is superior to suture.