压力对培养的人眼筛板细胞及胶原蛋白合成的影响

The influence of pressure on human lamina cribrosa cell and its collagen synthesis

目的研究压力对人眼筛板细胞形态学改变及胶原蛋白合成的影响。方法对筛板细胞进行体外培养，在光镜和电镜下观察压力对人眼筛板细胞形态和结构的影响，并使用显微荧光光度计对筛板细胞胶原蛋白合成进行定量测定。结果静压力６．６７ｋＰａ（１ｋＰａ＝７．５ｍｍＨｇ）下，筛板细胞向两端延长，形态变扁；随时间的延长，胞浆内空泡增多，线粒体肿胀、部分空泡化，并可见髓鞘样小体。加压３天的筛板细胞合成胶原蛋白Ⅰ、Ⅲ、Ⅳ型分别为４３．９５±６．３７、６１．３５±１０．３５、８２．９０±１１．３６荧光强度（ａｒｂｉｔｒａｒｙｕｎｉｔ，ＡＵ）与对照组比较，差异有显著性（Ｐ＜０．０５）。加压５天的筛板细胞合成胶原蛋白Ⅰ、Ⅲ、Ⅳ型分别为３３．５０±６．９４、４０．８５±１２．３０、８０．４５±１４．６５ＡＵ，与对照组比较仍有不同程度增高。结论压力引起筛板细胞形态的异常，并促使其合成胶原蛋白增加。

Objective To study the influence of pressure on human lamina cribrosa cell (HLC) and its synthesis of collagen. Method HLCs were cultured in vitro and submitted to elevated hydrostatic pressure, their changes in morphology were observed by light and electron microscopy and their synthesis of collagen was measured with fluorescence spectrophotometer. Results Under a hydrostatic pressure of 6.67 kPa (1 kPa=7.5 mmHg), the flat and polygonal HLCs became elongated. Vacuoles appeared in the cytoplasm and increased as the pressure persisted. The mitochondria were swollen, some of them turned into vacuoles, and myelin figures were found in the cytoplasm. When exposed to elevated pressure for 3 days, the amounts of synthesized collagen type I, III, IV were 43.95± 6.37, 61.35±10.35 and 82.90±11.36 arbitrary unit (AU) by fluorometry respectively, and compared to controls, they were statistically significantly ( P <0.05) increased; after 5 days of pressure exposure, the amounts were 33.50±6.94, 40.85±12.30 and 80.45±14.65 AU, e.g., still increased. Conclusion It is indicat ed that under pressure, HLCs undergo obvious morphological changes and increase their synthesis of collagen types I, III and IV.