Real-Time荧光PCR结合微量凝集试验早期诊断军团菌感染

Real-Time PCR combined with microagglutination test for early diagnosis of legionella infection

[目的]建立基于微量凝集试验和Real-Time荧光PCR两种方法上的军团菌早期诊断方法。[方法]对收集的疑似军团菌感染的血清标本配对同时进行微量凝集试验和Real-Time荧光PCR,比较两种方法的检测结果,并对结果加以解释。[结果]两种方法采用同样的标本但反映的信息各有不同,两种方法的结果有66.7%的符合率,两种方法的差异无统计学意义。[结论]微量凝集试验和Real-Time荧光PCR两种方法结合对临床军团菌病进行早期诊断具有标本易于获得,实验操作简单、快捷的优点。

[Objective] The aim of this study was to assess the combination of Real-Time PCR and microagglutination test as a rapid diagnostic method. [Methods] Serum samples were tested by methods of MAT and Real-Time PCR.The results were compared,and the consistence and difference explained. [Results] On the basis of processing the same sample,the two methods reflected different information separately.The concordance rate of MAT and Real-Time PCR was 66.7%,and the contradiction between the two could be well explained. [Conclusion] The sample used in the two methods was easy to be obtained,and the two methods were simple to be used with time saved.The two methods combined could be used as an early diagnostic method for Legionella.