骨肉瘤雌、孕激素受体的病理学研究

Pathological study of ER and PR in osteosarcoma

目的探讨雌激素受体（ＥＲ）、孕激素受体（ＰＲ）与骨肉瘤病理特征的关系。方法应用免疫组化ＳＰ方法（改良ＳＰ法）对６５例骨肉瘤蜡块组织进行了ＥＲ、ＰＲ检测。骨肉瘤的病理分型及分级按刘子君法划分。结果（１）阳性率：ＥＲ４０％（２６例），ＰＲ３２．３％（２１例）；（２）阳性颗粒分布于胞浆和胞核；（３）高分化组ＥＲ、ＰＲ阳性率高于低分化组，差异有显著性意义（Ｐ＜０．０５）；（４）病理分型与ＥＲ、ＰＲ阳性率无明显相关关系（Ｐ＞０．０５）。结论骨肉瘤瘤细胞中存在ＥＲ、ＰＲ，其阳性率与肿瘤分化程度呈正相关。

Objective To determine the relationship between estrogen receptor(ER),progesterone receptor(PR) and pathological characteristics of osteosarcoma. Method ER and PR were detected by streptavidin/peroxidase(SP) method immunohistochemically using the paraffin embedded tissues from 65 cases of osteosarcoma.The patients were typed and graded pathologically according to Liu Zijun's classification. Result The positive rates of ER and PR were 40%(26/65),32 3%(21/65) respectively. The positive particles were distributed in cytoplasm and nucleus.The positive rates of ER and PR in osteosarcoma with higher cell differentiation grade were higher than those in the tumor with lower one.The differences were statistically significant ( P <0 05 ).There were no relations to the pathological type. Conclusion ER and PR exist in osteosarcoma and their positive rates are related to the cell differentiation of osteosarcoma.