人原代晶体上皮细胞bFGF多肽和mRNA的表达

Expression of bFGF in primary human lens epithelial cells

目的为研究后发性白内障的发生机理，检测生长中人晶体上皮细胞（ｈｕｍａｎｌｅｎｓｅｐｉｔｈｅｌｉａｌｃｅｌｓ，ＨＬＥＣｓ）自身是否表达碱性成纤维细胞生长因子（ｂａｓｉｃｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ，ｂＦＧＦ）。方法体外培养人晶体上皮细胞，用免疫细胞化学和原位核酸分子杂交的方法，检测ＨＬＥＣｓ中ｂＦＧＦ多肽和其ｍＲＮＡ的表达。结果用免疫细胞化学和原位核酸分子杂交方法，可检测到生长中的人晶体上皮细胞自身表达碱性成纤维细胞生长因子。结论结合碱性成纤维细胞生长因子促进人晶体上皮细胞生长的作用，推测人晶体上皮细胞自身表达的ｂＦＧＦ。

Objective To study the mechanism of after cataract formation and investigate the expression of basic fibroblast growth factor(bFGF) in primary human lens epithelial cells(PHLECs). Methods Human lens epithelial cells were cultured in vitro, and the expression of bFGF was detected with immunohistochemistry and in situ hybridization.Results PHLECs express bFGF in protein and mRNA levels.Conclusion Based on bFGF promoting PHLEC growth and differentiation,the results suggest that bFGF from PHLECs take part in promoting such cell growth and differentiation.