高脂血症大鼠肠系膜上动脉NPY免疫反应性神经纤维的变化

CHANGES OF NPY CONTAINING INNERVATION ON THE WALL OF THE SUPERIOR MESENTERIC ARTERIES IN HYPERLIPIDEMIC RATS

为探讨神经肽Ｙ与动脉粥样硬化的关系，用ＡＢＣ免疫组织化学及体视学方法观察高脂血症大鼠肠系膜上动脉神经肽Ｙ免疫反应性神经纤维的变化。（１）定性观察：对照组各时期神经肽Ｙ免疫反应性神经纤维呈网状攀附于血管周围，分布均匀，并见串珠状膨体；高脂组纤维呈密集网状分布，在纤维网孔中出现大量较纤细的新增生纤维，其排列较紊乱，膨体清晰可见，这种变化见于各期实验组。（２）定量结果：对照组各时期纤维密度无明显改变；高脂组纤维密度逐渐增多，３０ｄ、９０ｄ与７ｄ比较差异显著（Ｐ＜０．０１），且分别较对照组增多（Ｐ＜０．０１）。结果提示，血脂升高可促使肠系膜上动脉壁神经肽Ｙ免疫反应性神经纤维增生，这种变化可能与动脉粥样硬化的发生。

For studying the relationship between neuropeptide Y and atherosclerosis, the NPY containing innervation on the wall of the superior mesenteric arteries in hyperlipidemic rats was examined by ABC immunohistochemical and morphometric technique. The results were as follows: (1)qualitative observation: on the wall of the mesenteric arteries there were a number of reticular innervations with strings of varicosities, which distribution was regular, in the control group; The distribution of NPY containing innervations in hyperlipidemic group were denser reticular pattern, and the newborn NPY containing innervations, which also had varicosities, appeared in the reticular innervations; Their distributions were irregular. These changes could be observed in all periods in experimental groups. (2)quantitative results: in the control group, the density between two of them was not significantly different, but in the hyperlipidemic group, the density of NPY containing innervations increased significantly at 30d and 90d, compared with that at 7d( P <0 01) or with that in the control group ( P <0 01). The results suggested that hyperlipidemia should result in the increase of the NPY containing innervation, which might be associated with the development of atherosclerosis.