人外周血树突状细胞在LAK抗HPBALL细胞中作用的研究

THE STUDY OF FUNCTION OF HUMAN PERIPHERAL BLOOD DENDRITIC CELLS IN THE SYSTEM OF LAK ANTI HPBALL CELLS IN VITRO

为探讨树突状细胞（ＤＣ）在ＬＡＫ抗ＨＰＢＡＬＬ细胞中的作用，采用多因素、多水平的杀伤试验，同时以光镜、电镜观察ＤＣ、ＬＡＫ、ＨＰＢＡＬＬ相互作用的形态特征及ＤＮＡ断端标记法检测瘤细胞是否凋亡。结果表明：（１）ＤＣ无直接杀伤ＨＰＢＡＬＬ作用。（２）５×１０５～１×１０７／ＬＤＣ有增强不同Ｅ／ＴＬＡＫ杀伤活性的趋势，而１×１０７～５×１０７／ＬＤＣ对ＬＡＫ活性有抑制趋势。（３）ＤＣ、ＬＡＫ杀伤ＨＰＢＡＬＬ的最佳组合条件为：ＤＣ培养４ｄ、浓度５×１０６／Ｌ，ＬＡＫＥ／Ｔ＝１０／１，ｒＩＬ－２＝０。（４）光镜、电镜下均可见ＤＣ的突起与ＬＡＫ、ＨＰＢＡＬＬ细胞紧密接触形成细胞簇。（５）ＤＮＡ断端标记法显示瘤细胞呈末端脱氧核糖核酸转移酶阳性反应。

Factorial design and orthogonal design were adopted to examine the function of dendritic cells(DC)in the system of lymphokin activated killer cells (LAK) anti HPBALL tumor celler.In the mean time,the interactive morphological character of DC,LAK and HPBALL cells was observed under light and transmission electron microscope,and programmed cell deaath assay was applied to research whether or not HPBALL cells had been triggered to apoptosis. The results were as follows:(1)DC could not kill HPBALL cells directly.(2)5×10 8 to 1×10 7/L DC could have a trend of enhancing the killing activity of different effector/target(E/T)LAK cells.However,1×10 7/L to 5×10 7/L DC showed an inhibitory trend.(3)The most optimal combined elements of DC,LAK for killing HPBALL cells was:DC cultured for 4 days concentration 5×10 6/L,E/T=10/1 LAK without IL 2.(4)It was also observed that DC could contact and cluster with LAK and HPBALL cells.(5)The positive reaction of programmed cell death apperaed in HPBALL cells. The results indicated that DC could regulate the cytotoxity of LAK killing HPBALL cells in some degree.