大鼠胃窦部胃泌素和生长抑素mRNA及其相关蛋白表达的研究

EXPRESSION OF GASTRIN,SOMATOSTATIN AND THEIR CORRESPONDING mRNA IN RAT ANTRUM

为探讨大鼠胃窦部胃泌素ｍＲＮＡ和生长抑素ｍＲＮＡ在Ｇ、Ｄ细胞的转录及其相关蛋白的表达，用免疫细胞化学和原位杂交技术，检测了大鼠胃窦部Ｇ细胞内的胃泌素和Ｄ细胞内的生长抑素以及它们相应的ｍＲＮＡ。结果显示，大鼠胃窦部的Ｇ、Ｄ细胞位于幽门腺基部，细胞分布不均，单个或多个在一起；胃泌素和生长抑素均匀地分布于胞质内，核内阴性；Ｇ／Ｄ细胞比值在１．３±０．３２～１．５５±０．７５之间，即Ｇ细胞多于Ｄ细胞。ｍＲＮＡ信号染色强度细胞间有差异，呈极性分布，多位于核周或核上胞质内。ｍＲＮＡ阳性细胞数在单位面积内少于Ｇ、Ｄ免疫组织化学显示阳性细胞数，原因可能是由于多聚甲醛的固定对ｍＲＮＡ的降解，降低了ｍＲＮＡ的活性，从而导致某些Ｇ、Ｄ细胞内ｍＲＮＡ不能被检出。该法具有安全、省时、定位准确等优点。

In situ hybridization and immunocytochemical technique have been used to detect the gastrin,somatostatin and their corresponding mRNA in G,D cells of rat gastric antrum.The results showed that G,D cells one or three cells together located in basal part of pyloric glands,Gastrin and somatostatin distributed homogenously in cytoplasma,but the nuclei were negative.The number of G cells was more than that of D cells,and their ratio was ranged from 1 3±0 32 to 1 55±0 75.The staining intensity of mRNA signal was different in individual cells,and exhibited a polarized distribution either located in peripheral cytoplasm or in supernuclear cytoplasma.The number of mRNA positive cells was constanty lower than that of the G or D immunoreactive cells in an unit area.It was probably caused by the degradation of mRNA through the paraformaldehyde fixation which failed to detect the specific mRNA in some G,D cells.This method has many advantages including fast,safety,and precise localization.It can be used in general laboratory and clinical diagonosis.