大蒜素诱导人胆管癌细胞QBC939凋亡的实验研究

Induction of apoptosis in human cholangiocarcinoma cell line QBC939 by allicin in vitro

目的:观察大蒜素对人胆管癌细胞QBC939的作用。方法:MTT法检测大蒜素对QBC939细胞的抑制作用;光镜和电镜观察大蒜素作用后细胞形态的变化;流式细胞仪分析细胞周期的改变;凝胶电泳法检测细胞凋亡情况。结果:随着大蒜素作用时间的延长及浓度的增加,其对QBC939细胞的增殖抑制作用就越明显;流式细胞仪分析显示G2/M期阻滞和细胞凋亡;凝胶电泳示实验组DNA梯带(DNA ladder)形成。结论:大蒜素诱导的人胆管癌细胞的凋亡与细胞DNA分裂期阻滞密切相关。这一作用机制将为临床治疗提供理论依据。

Objective：To study the effect of allicin on human cholangiocarcinoma QBC939 cells. Methods：The cell proliferation was assessed by MTT assays; the morphological changes of the QBC939 cells were observed under light and electron microscopes;cell cycle kinetics and apoptosis were analyzed by flow cytometry and microscopic and DNA extraction and electrophoresis examination.Results：Allicin inhibited the cell growth in concentration and time dependent manners.The cell showed G2/M arrest and apoptosis; the typical ladder pattern fragmention were observed DNA gel electrophoresis. Conclusion：Allicin suppresses the growth of QBC939 cells in vitro by causing cell cycle arrest and apoptosis of the cells.The mechanisms of allicin will provide theoretical guidance for clinical treatment.