摘要
目的:探讨低分子肝素对胃癌SGC-7901细胞株的生长以及其血管内皮生长因子(VEGF)表达的影响。方法:采用MTT方法观察一定浓度速碧林(低分子肝素钙注射液)作用于SGC-7901细胞12h,24h,48h后细胞的生长情况。用逆转录聚合酶链反应(RT-PCR)分析SGC-7901细胞VEGF mRNA表达的变化,同时用酶联免疫吸附实验(ELISA)检测细胞上清中VEGF的蛋白表达量。结果:速碧林对SGC-7901细胞的生长有抑制作用,且呈剂量和时间依赖性;与对照组相比,速碧林能降低SGC-7901细胞VEGF mRNA以及蛋白的表达。结论:低分子肝素能抑制SGC-7901细胞的生长以及VEGF的分泌。
Objective:To explore the effect of Low-Molecular-Weight-Heparins on cell proliferation and the expression of vascular endothelian growth factor(VEGF) in human gastric cancer cell line. Methods : SGC-7901 cells were cultured in 1640 medium supplied with different concentration of Low-Molecular-Weight-Heparins. The proliferative activity of SGC-7901cells were evaluated by MTT assay. The expression of VEGF in the cells were determined by RT-PCR and enzyme-linked immunosorbent assay(ELISA). Results :Cell proliferation were inhibitated in time dependent and dose-dependent, the expression of VEGF were reduced in the cells treated with Low-Molecular-Weight-Heparins. Conclusion : Low-Molecular-Weight-Heparins can inhibit the proliferation of SGC-7901 cells and the expression of VEGF.
出处
《陕西医学杂志》
CAS
2010年第4期399-400,427,共3页
Shaanxi Medical Journal
基金
江苏省无锡市科技局项目
关键词
胃肿瘤/免疫学
血管生成因子/分析
内皮
血管
肝素
低分子量
@细胞株
Stomach neoplasms/immunology Angiogenesis factor/analysis Endothelium
vascular Heparin
low-molecular-weight @SGC-7901