银杏成熟胚培养的细胞组织学观察

THE MATURE EMBRYO OF GINKGO BILOBA L: IN VITRO CULTURE AND ITS CYTOHISTOLOGICAL STUDIES

由银杏（ＧｉｎｋｇｏｂｉｌｏｂａＬ．）成熟胚诱导的淡绿色、疏松愈伤组织经过继代培养后在部分疏松愈伤组织上又形成致密愈伤组织，并在ＭＳ＋ＢＡ２ｍｇ／Ｌ＋ＮＡＡ０５ｍｇ／Ｌ的培养基上从致密愈伤组织中诱导胚状体发生并达到心形胚阶段。细胞组织学的观察表明，胚状体起源于愈伤组织表面的胚性细胞。

Callus derived from completed embryos of Ginkgo biloba L. were light green and crisp. And after subculture, some callus changed from crispy to compact. In MS medium supplemented with 2mg/L BA and 05mg/L NAA, somatic embryos were obtained from the compact callus. Embryoids originated from embryonic cell at the edge of callus, and embryoids at whatever stage (globular or heart) exhibited a slow growth, which delayed transfer onto subculture medium.