顺铂诱导人卵巢癌AO 10/17细胞凋亡的实验研究

Experimental Study of Apoptosis Induced by Cisplatin in Human Ovarian Carcinoma AO 10/17 Cells

目的探讨顺铂是否通过诱导肿瘤细胞凋亡而抑制肿瘤生长，以及细胞凋亡和细胞周期、细胞内外钙离子浓度的关系。方法应用顺铂处理ＡＯ１０／１７细胞，ＨＥ染色观察细胞形态学改变；琼脂糖凝胶电泳观察ＤＮＡ降解，以及应用流式细胞仪观察细胞凋亡过程中细胞周期的改变及细胞内钙离子的变化。结果在顺铂作用下，ＡＯ１０／１７细胞呈凋亡改变，ＤＮＡ琼脂糖凝胶电泳呈典型的凋亡特征。细胞凋亡的同时细胞周期发生特定的改变，细胞内钙离子升高，但用ＣａＣｌ２与顺铂同时处理细胞，没有促进顺铂的作用。结论顺铂通过细胞内钙离子变化，诱导人卵巢癌细胞凋亡，是顺铂抑制卵巢肿瘤生长的机理之一；

Objective Whether apoptosis induced by cisplatin resulted in tumor growth inhibition and the relationships between apoptosis and cell cycle, intracellular and extracellular Ca++ concentration were investi gated. Methods AO 10/17 cells were treated with cisplatin. Morphological changes (HE stain) were observed under light microscope; DNA degradation was assayed by electrophoresis on agarose gel. The change of cell cycle was analyzed by flow cytometer and cytosolic Ca++ concentrations were monitored using probe of calcium ion fluo3/AM. Results Under the influence of cisplatin, AO 10/17 cells exhibited changes of apotosis both in morphology and in the characteristics of electrophoresis. Meanwhile the cell cycle also showed special change and intracellular Ca++ concentration was increased. Increase in extracellular Ca++ concentration when AO 10/17 cells were treated with CaCl2 and cisplatin did not enhance the efficacy of cisplatin. Conclusion The results suggest that apoptosis followed by change of cytosolic Ca++ concentration is one of the mechanism of cisplatin in inhibition of ovarian tumor growth. It is closely related to cell cycle.