增殖性玻璃体视网膜病变组织中炎性细胞因子的检测

A preliminary study on cytokines in proliferative vitreoretinopathy

目的检测白细胞介素６（ｉｎｔｅｒｌｅｕｋｉｎ６，ＩＬ６）、肿瘤坏死因子（ｔｕｍｏｒｎｅｃｒｏｓｉｓｆａｃｔｏｒ，ＴＮＦ）等炎性细胞因子在增殖性玻璃体视网膜病变组织和玻璃体液中的水平。方法用免疫组织化学方法检测视网膜前膜沉积的ＩｇＧ和补体Ｃ３；用ＥＬＩＳＡ方法测定３３只增殖性玻璃体视网膜病变（ｐｒｏｌｉｆｅｒａｔｉｖｅｖｉｔｒｅｏｒｅｔｉｎｏｐａｔｈｙ，ＰＶＲ）眼和８只正常眼玻璃体液中ＩｇＧ、补体Ｃ３的含量；用考马斯亮蓝法测定玻璃体总蛋白；采用待测标本对ＩＬ６依赖细胞株增殖反应的影响测定玻璃体液中ＩＬ６的相对活性；用对Ｌ９２９肿瘤细胞的杀伤作用检测玻璃体液中ＴＮＦ的生物活性。结果ＰＶＲ眼视网膜前膜有大量ＩｇＧ、补体Ｃ３沉积；ＰＶＲ眼玻璃体液中ＩｇＧ、补体Ｃ３、玻璃体总蛋白含量均明显高于对照组；正常对照眼玻璃体液中未检出ＩＬ６和ＴＮＦ活性，１７只ＰＶＲ眼玻璃体液中有１３只检出ＩＬ６活性，１１只ＰＶＲ眼玻璃体液中有３只检出ＴＮＦ活性；玻璃体液中ＩＬ６活性增高与ＰＶＲ病情程度有显著的相关性。结论视网膜前膜有ＩｇＧ和补体Ｃ３的沉积，ＰＶＲ眼玻璃体液中ＩｇＧ、补体Ｃ３含量增加，ＩＬ６及ＴＮＦ活性的检出?

Objective To investigate the roles of cytokines like interleukin 6 (IL 6), tumor necrosis factor (TNF) and local immune reaction in proliferative vitreoretinopathy (PVR). Methods Immunohistochemical procedures were applied to identify IgG and complement C 3 deposits in the specimens of epiretinal membranes surgically obtained from patients with PVR. Vitreous aspirates from 33 eyes with idiopathic and traumatic PVR and 8 normal eyes were examined for IgG and complement C 3 by using ELISA; the total vitreous protein (TVP) was determined by Coomassie Brilliant Blue Test; the relative activity of IL 6 in vitreous was tested by means of a proliferative response of IL 6 dependent cells to vitreous samples; and the biological activity of TNF in vitreous was measured with its killing effect on L 929 tumor cells. Results Diffuse deposits of IgG and C 3, TVP in epiretinal membanes of PVR eyes were significantly higher than those in the control group; the activities of IL 6 and TNF (0/8) were not detected in any of the normal control, IL 6 was detected in 13 of 17 vitreous samples and TNF was detected in 3 of 11 vitreous samples from PVR eyes; and vitreous IL 6 activity was found to be positively correlated with the severity of PVR. Conclusion The deposits of IgG and C 3, their elevation in amount and the detection of IL 6 and TNF activity in the vitreous samples from patients with PVR suggest that the cytokines and local immune reaction play a role in the pathogenesis of PVR.