B7-H4蛋白在上皮性卵巢癌中的表达及临床意义

EXPRESSION OF B7-H4 PROTEIN IN OVARIAN EPITHELIAL CANCER AND ITS CLINICAL SIGNIFICANCE

目的探讨B7-H4蛋白在上皮性卵巢肿瘤中的表达及临床意义。方法用链毒菌抗生物素蛋白过氧化物酶免疫组织化学染色的方法检测B7-H4蛋白在正常卵巢、良性上皮性卵巢肿瘤、交界性上皮性卵巢肿瘤、上皮性卵巢癌组织中的表达,并分析其与各临床病理特征的关系。结果 B7-H4蛋白在卵巢癌组阳性表达率显著高于正常组、良性组、交界组,差异有统计学意义(P<0.01),其余各组间差异无统计学意义(P>0.05)。B7 H4的表达与病理分期、分级、类型相关,差异有统计学意义(P<0.05)。上皮性卵巢癌分期越晚、分化越差B7 H4表达越高,浆液性癌B7-H4表达高于黏液性癌。结论 B7-H4在卵巢癌中的高表达及其与病理类型、临床分级、分期的关系,提示其可能与肿瘤发生发展有关,可为卵巢恶性肿瘤诊断及治疗提供靶位点。

Objective To investigate the expression of B7-H4 protein in ovarian tumors and its clinical significance. Methods The expression of BT-H4 protein was detected by immunohistochemical streptavidin-peroxidase （SP） method in normal ovarian tissues, benign epithelial ovarian tumors, borderline ovarian tumors and ovarian carcinoma. The relationship between BT-H4 protein and pathological feature was analyzed. Results The positive expression rate of BT-H4 protein in ovarian carcinoma group was higher than normal, benign, and borderline group （P〈 0.01）. No significantly statistical difference was found between other two-two comparisons （P 〉 0.05）. The expression of BT-H4 protein Was correlated with pathological stage,grade and type （P 〈0.05）. Tissues from ovarian carcinoma of late stage and poor differentiation presented with increased B7-H4 protein expression, the positive expression rate of BT-H4 protein in serous cystadenocarcinoma was higher than mucinous cystadenocarcinoma. Conclusion The up-regulation of B7-H4 protein in ovarian carcinoma has positive relationship with late stage and lower grade,reveals that B7-H4 may play an important role in the genisis and development of ovarian cancer. The detection of B7-H4 protein expression may be helpful in malignant ovarian tumor diagnosis and treatment.