小儿与老年白内障晶体上皮细胞密度及增殖细胞核抗原表达

Cell density and expression of proliferating cell nuclear antigen (PCNA) in the lens epithelium of children and aging patients with cataract

目的检测儿童和老年人晶体上皮细胞密度和增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｎｇｃｅｌｎｕｃｌｅａｒａｎｔｉｇｅｎ，ＰＣＮＡ）表达，探讨晶体上皮细胞与白内障术后后囊混浊的关系。方法用ＨＥ染色观察白内障小儿组（＜１２岁）及老年组（５１～８０岁）中央区晶体上皮铺片的细胞密度，用免疫组化染色及真彩色医学图像分析系统检测ＰＣＮＡ的表达及积分吸光度（Ａ）值。结果晶体上皮细胞密度在小儿组为５０２０．２５±２４６．０１个／平方毫米，老年组为４３４０．００±２４０．９５个／平方毫米（Ｐ＜０．０５）。ＰＣＮＡ积分吸光度（Ａ）值在小儿组为２．８９±０．５７，老年组为２．１３±０．６３（Ｐ＜０．０５）。结论小儿组晶体上皮细胞密度和积分吸光度（Ａ）值明显高于老年组，提示可能为小儿后发性白内障高发的重要因素。

Objective To determine the cell density and expression of proliferating cell nuclear antigen (PCNA) in the lens epithelium of children and aging patients with cataract. Methods The lens epithelial cells (LECs) of the central area were examined in two groups, children (less than 12 years old) and senile persons (51～80 years old). The densities of LECs in the two groups were calculated with samples stained with hematoxylin and eosin (HE) staining, and the PCNA was tested immunohistochemically. The integral optical density (IOD) of PCNA expression was measured through a medical color image analysis system. Results The densities of LECs in children and senile persons were 5 020.25±246.01 cells/mm2 and 4 340.00±240.95 cells/mm2 (P<0.05), the IODs were 2.89±0.57 and 2.13±0.63 (P<0.05) respectively. Conclusion The LEC counting and IOD values in children eyes are significantly higher than that of senile persons, suggesting that they be one of the important factors in the formation of posterior capsular opacification (PCO) or after cataract in children eyes.