包埋后的几丁质与软骨细胞体外培养的实验研究

Culture of chondrocytes seeded onto chitin scaffoldings coating with different materials: experimental study

目的探讨几丁质作为组织工程技术中细胞培养支架的可行性。方法采用聚乳酸、卵磷脂及多聚赖氨酸分别或共同包埋几丁质与软骨细胞体外培养，观察其亲水性的改变、对细胞吸附力和细胞功能的影响。结果以聚乳酸包埋的几丁质对细胞的生长有抑制作用；以卵磷脂包埋的几丁质亲水性增强；以多聚赖氨酸包埋的几丁质对细胞吸附力增强；以卵磷脂和多聚赖氨酸共同包埋的几丁质具有良好的亲水性和对细胞吸附力，并可使细胞更好地发挥功能。结论聚乳酸。

Objective To find the possibility of chitin used as scaffolds in tissue engineering.Method Chondrocytes were cultured when they were seeded onto chitin meshcoated with polylactic acid (PLA) lecithin (LEC) and polyllysine(PLYS).With light microscope and scanning electron microscope, the scaffolds in hydrophilia and adsorptivity to chondrocytes and the function of the cells were observed. Result LEC can change the hydrophilia of scaffolds. PLYS can strengthen the adsorptivity of the scaffolds. and promote the matrices production of chondrocytes. Conclusion The chitin coated with LEC and PLYS can be used as scaffolds in tissue engineering.