脱钙牙本质基质及其复合物整复节段性骨缺损的组织学观察

Histological Study of Decalcified Dentin Matrix and Its Composite in Reconstruction of Segmental Bone Defect

采用随机同期对照方法对成年家兔桡骨节段性缺损模型，分别植入自制脱钙牙本质基质、生物活性玻璃陶瓷加脱钙牙本质基质的复合物、脱钙骨基质整复骨缺损，行大体及组织学观察。结果发现，用脱钙牙本质基质整复的骨缺损区在术后８周即可以达到骨愈合，髓腔再通，整复节段性骨缺损的速度和质量与脱钙骨基质相似，而生物活性玻璃陶瓷加脱钙牙本质基质复合物整复区在８周时达到骨性愈合，但髓腔未能再通。空白对照在术后８周至１２周未达到骨性愈合，仅见缺损区有断续的小梁样骨形成。

The study was designed to investigate the histological differences of decalcified dentin matrix(DDM) and its composite of DDM+BGC and decalcified bone matrix(DBM)in reconstruction of segmental defect in radius. Segmental bone defect models in radius of rabbit were employed,and DDM, DBMand DDM+BGC materials were implanted in the left and right defect regions of 48 New Zealand Rabbits(divided into 2 groups,subdivided into 6 subgroups for each group)respectively. DBM group was taken as standard control while blank control was also taken in the study.Grossly among DDM, DBM, DDM+BGC groups, there was no obvious difference at the end of 12 weeks. HE stain showed that at the end of 12 weeks after operation, the three groups had almost the same new bone formation in quality and quantity, and ununions presented in all cases of the blank control groups and only pieces of separate trabecular bone particles presented in the defect. Conclusively, DDM is functionally equal to DBM in reconstruction of segmental bone defect while DDM+BGC demonstrates less value than DBM when concered with medullary reconstruction.