脱钙牙本质基质及其复合物整复节段性骨缺损的X线及新骨钙磷动态变化研究

X-ray Observation and Ca/P Quantitative Analysis of Decalcified Dentin Matrix and Its Composite in Reconstruction of Segmental Bone Defect

采用完全随机同期对照方法，对家兔桡骨节段性缺损动物模型，分别植入自制脱钙牙本质基质（ＤＤＭ）、生物活性玻璃陶瓷（ＢＧＣ）与脱钙牙本质基质复合物、脱钙骨基质（ＤＢＭ）整复骨缺损，行Ｘ线观察及新骨钙磷元素动态变化的测定。结果显示：用ＤＤＭ整复的骨缺损区在术后各程期的Ｘ线表现与ＤＢＭ相似，第８周时新旧骨界线消失，髓腔再通；而ＤＤＭ加ＢＧＣ的骨整复区在第８周时虽然骨断端新旧骨的界线消失，但新骨的Ｘ线表现仍为一阻射团块；空白对照侧在术后８～１２周Ｘ线表现为骨断端成锥形，断端间无骨性愈合。新骨Ｘ线能谱分析结果显示，ＤＤＭ和ＤＢＭ组内各程期Ｃａ／Ｐ值有显著性差异（Ｐ＜０．０１）；除ＤＤＭ及ＤＢＭ第１２周时的Ｃａ／Ｐ值外，３种材料各程期新骨Ｃａ／Ｐ原子个数比与正常骨相比有显著性差异（Ｐ＜０．

A model of segmental bone defect in radius of rabbit was employed, and DDM,DBM and DDM+BGC were implanted in the left and right defect regions of 48 New Zealand rabbits respectively.The samples were sacrificed in 1,2,3,4,8,12 weeks after operation,then X ray examination and electromicroscopic Ca/P atom amount ratio measurement were performed.The results showed that DDM group and DBM group presented a completely medullary reconstruction while DDM+BGC group did not demonstrate reconstruction of medullary cavity 8 weeks postoperatively. The three kinds of materials showed the good reconstruction capacities of bone defect,and Ca/P analysis was also in cooperation with the X ray observations.