感染华支睾吸虫的豚鼠胆道扫描电镜观察

A scanning electron microscopic observation of the bile duct in guinea pigs infected with clonorchis sinensis

目的：研究华支睾吸虫病的胆道超微结构变化及其病变机理。方法：以实验感染华支睾吸虫的豚鼠为模型，分别于感染后第６、１０、１４、１８及２２周取其肝内胆管以扫描电镜进行观察。结果：主要变化有：肝内胆管上皮细胞微绒毛的变短，肿大或消失；细胞膜表面的大疱形成；腔面质膜的破裂、缺损；细胞间隙增宽及胆管上皮细胞的局灶性脱落；粘液分泌亢进。结论：胆管上皮细胞的病损程度随感染时间的延长呈加重趋势。

Purpose To study the ultrastructural changes and the pathological mechanism of the bile duct with clonorchiasis. Methods The guineas pigs were used as experimental model infected with clonorchis sinensis. The intrahepatic bile ducts of the guinea pigs were observed with scanning electron microscopy (SEM) at 6th, 10th, 14th, 18th, 22nd weeks after infection. Results The major changes were shortening, odema or loss of the microvilli of intrahepatic bile duct epithelial cells (IBDECs); bleb formation of the surface of the membrane of IBDECs; broken or loss of the cytoplasmic membrane facing to lumen; widening of intercellular space and local slough of IBDECs; active secretory of the mucus. Conclusion The results show that the extents of pathological changes tend to be more severe while the time follows up. It also shows that the lesion of IBDECs and functional promotion take place alternatively.