一株微生物絮凝剂产生菌的絮凝特性及处理气田废水研究

Analysis on Optimal Culture Conditions and Characteristics of A Flocculant-producing Bacterial Strain

从土壤中分离、筛选得到一株稳定高效的微生物絮凝剂产生菌,命名为B—27。考察了碳源、氮源、pH、温度、培养时间等多种因素对絮凝剂(命名MBF—27)絮凝效果的影响。实验结果表明,当碳源为葡萄糖、氮源为酵母膏、pH为7.0、温度为35℃、培养时间为72h时,絮凝剂MBF—27对高岭土悬浊液的絮凝率可达93.8%。红外光谱扫描分析表明,该絮凝剂的主要成分为多糖类物质。同时,生物絮凝剂MBF—27对气田废水的COD和色度去除率分别达到60.77%和86.1%,优于传统的絮凝剂聚乙烯丙胺(PAM)和三氯化铁(FeCl3)。

A floeculant - producing bacterial strain named B-27 was islated from the soils. This research was focused on the optimal culture conditions such as carbon source, nitrogen source, pH, temperature and culture time. The results were as follow： The optimal flocculating rate of MBF-27 was up to 93.8% when the carbon source,nitrogen source,pH, temperature and culture time were glucose, yeast extract, pH 7.0,35℃, 72h respectively. Meanwhile, the infrared spectra showed that the main ingredient of bioflocculant MBF-27 was polysaccharide. The COD and Chroma removal of MBF-27 were 60.77 % and 86.1% respectively, which was better than that of PAM and FeCl3.