食管鳞状细胞癌中人乳头状瘤病毒感染的意义

Significance of human papillomavirus (HPV) infection in squamous cell carcinomas of the esophagus

目的：探讨人乳头状瘤病毒（ＨＰＶ）感染在食管鳞状细胞癌（ＥＳＣＣ）发生发展中的意义。方法：采用原位杂交和免疫组化ＬＳＡＢ法检测３３例ＥＳＣＣ中的ＨＰＶＤＮＡ、ＨＰＶ属特异性结构抗原（ＨＰＶＧＳＡｓ）和增殖细胞核抗原（ＰＣＮＡ）。结果：３３例ＥＳ－ＣＣ中的８例可见ＨＰＶＤＮＡ阳性细胞，阳性率达２４．２％；这些ＨＰＶＤＮＡ阳性细胞的ＰＣＮＡ均阴性，３３例ＥＳＣＣ，包括８例有ＨＰＶＤＮＡ阳性细胞者，ＨＰＶＧＳＡｓ均为阴性。在癌旁增生、异型增生和原位癌灶中未见ＨＰＶＤＮＡ或ＨＰＶＧＳＡｓ阳性细胞。

Purpose To investigate the significance of papillomavirus (HPV) infection in the development and progression of the esophageal squamous cell carcinoma (ESCC).Methods The HPV DNA, HPV genus specific structural antigens (HPV GSAs) and proliferating cell nuclear antigen (PCNA) were detected in 33 ESCCs by use of in situ hybridization and LSAB immunohistochemistry, respectively. Results The HPV DNA positive cells, which were PCNA negative, could be found in 8 out of 33 ESCCs, the positive rate was 24 2%. No HPV GSAs positive cells were found in all these 33 ESCCs, including the 8 ESCCs with HPV DNA positive cells. Both HPV DNA and HPV GSAs were not presented in paracancerous lesions of hyperplasia, dysplasia or carcinoma in situ. Conclusion The HPV infection might play its role in the progression of some ESCCs.