人大肠癌细胞系HR-8348在鸡胚体内侵袭和转移的连续性观察

A series of studies on invasion and metastasis on human colorectal carcinoma cell line HR 8348 in the chick embryo

目的：模拟体内情况，研究大肠癌细胞对上皮层、间质、血管壁的连续性侵袭过程及转移特性。方法：将人大肠癌细胞系ＨＲ－８３４８接种至鸡胚绒毛尿囊膜上，分别在接种后的６、１２、２４ｈ及第２、３、５、７、９天对生长良好的瘤体组织及鸡胚有关器官取材，同时进行组织学、透射电镜、冷冻割断扫描电镜系列观察。结果：癌细胞对上皮层的侵袭是依其主动变形运动为基础，以团块（巢）状、弥散状和混合型３种方式进行的；在对间质的侵袭过程中，条索状较弥散状侵袭更常见；瘤细胞经血管壁进入血流的方式有两种：单个释放入血和以瘤细胞群的方式侵入血流。瘤细胞胞体高度变形后，伸向周围组织内、长而不规则的胞浆突起，对侵袭具有重要意义。结论：用此模型可理想地研究大肠癌对上皮层、间质和血管壁的连续侵袭过程。

Purpose To research the successive invasion and metastasis characteristics of human colorectal carcinoma in epithelial layers, stromal tissues and blood vessels as the neoplasm do in human body. Methods The human colorectal carcinoma cell line HR 8348 was transplanted on the chick embryos chorioallantoic membrane (CAM), after transplantation, these implanted tumors were cut off wholly at 6、12、24 hours and 2、3、5、7、9 days respectively, and inspected by LM、SEM and freeze fracture SEM. Results The transepithelial layers are based on active demormal movement of neoplastic cells and carried out by three pattern of cluster, sporadic and mixed. In the tumor invasion process to stromal tissues, the cord or cluster pattern is more seen than the sporadic ones. Blood vessels are invaded by two patterns, namely single and a cluster of cells. In this experiment, it is found that long and irregular cytoplasmics of the active invasive tumor cells, which were formed by highly deformation and protruded into the surrounding tissues, play an important role in the invasion processes. Conclusion This animal model can be used to study the successive invasion and metastasis processes, patterns and mechanism of large intestine carcinoma to epithelial layers, stromal tissues and blood vessels.