摘要
目的观察胰岛素样生长因子-Ⅰ(IGF-Ⅰ)在体外对人卵巢颗粒细胞雌二醇(E2)和孕酮(P)产生量及超微结构的影响,及其与促卵泡激素(FSH)的关系。方法培养人卵巢颗粒细胞,以终浓度为0.13、1.31、6.53和13.07nmol/L的IGF-Ⅰ及6.53nmol/L的IGF-Ⅰ+0.15nmol/L的FSH分别对其刺激,对照组不加IGF-Ⅰ及FSH,用放射免疫法检测颗粒细胞E2和P产生量,同时对IGF-Ⅰ浓度为6.53nmol/L组及对照组颗粒细胞作透射电子显微镜检查。结果E2产生量分别为43.9、59.5、87.3和92.8pmol/1000细胞(对照组为30.1pmol/1000细胞),P产生量分别为3.4、7.6、13.9和14.6nmol/1000细胞(对照组为1.0pmol/1000细胞),均明显高于对照组(P<0.01)。IGF-Ⅰ和FSH共同刺激后,E2和P产生量较单纯FSH刺激分别增加3.5倍和14.5倍(P<0.01)。经IGF-Ⅰ刺激后,颗粒细胞超微结构与对照组比较有明显差异,主要表现为线粒体和滑面内质网非常丰富,胞质内有大量脂滴。结论经IGF-Ⅰ刺激后,颗粒细胞超微结构向有利于雌、孕?
Objective To observe the effect of insulinlike growth factorⅠ (IGFⅠ) on steroidogenesis and ultrastructures of cultured human granulosa cells in vitro and the synergetic influence of follicle stimulating hormone (FSH) on this process. Methods Human granulosa cells were cultured in serumfree 1640 medium. After stimulated with various concentrations of IGFⅠ or IGFⅠ+FSH for 24 hours, estradiol (E2) and progesterone (P) levels in the media were measured by radioimmunoassay, and the ultrastructure changes of granulosa cells were observed under electronic microscope. Resutls When stimulated with different concentrations of IGFⅠfor 24 hours, E2 and P levels produced by granulosa cells were in a dosedependent manner and significantly higher than those of controls (P<0.01). The E2 and P levels in the media under the stimulation of IGFⅠ+FSH were 3.5 times and 14.5 times respectively higher than those of FSH alone. The main ultrastructureal appearences of granulosa cells stimulated with IGFⅠwere abundance of enlarged smooth enodplasmic reticulum, mitochondria and lipid droplets in cytoplasm. Conclusions IGFⅠ possesses a positive effect on the steroidogenesis of cultured human granulosa cells in dosedependent fashion which could be enhanced by combination of IGFⅠ and FSH. The ultrastructures of granulosa cells showed a tendency toward activation of steroidogenesis under the stimulation of IGFⅠ.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
1998年第9期546-548,I016,共3页
Chinese Journal of Obstetrics and Gynecology
关键词
IGF-Ⅰ
粒层细胞
卵巢
雌二醇
孕酮
Insulinlike growth factor I
Granulosa cells
Estradiol
Progesterone