面口部炎症刺激增强CGRP mRNA、PPTA mRNA及NOS在大鼠三叉神经节中的表达

INCREASED EXPRESSION OF CGRP mRNA,PPTA mRNA AND NITRIC OXIDE SYNTHASE IN RAT TRIGEMINAL GANGLION AFTER CARRAGEENAN INDUCED INFLAMMATION

为了研究ＣＧＲＰｍＲＮＡ、ＰＰＴＡｍＲＮＡ和ＮＯＳ在伤害性刺激后大鼠ＴＧ中合成和表达的变化，将４％鹿角菜胶（ＣＡＲ）溶液注射到大鼠一侧口周皮下作为伤害性刺激模型，用ＮＡＤＰＨ－ｄ组织化学法结合ＣＧＲＰ、ＰＰＴＡｍＲＮＡｓ原位杂交组织化学法对此进行了观察。结果显示：对照组大鼠ＴＧ中约有３２．２％、１６．２％和１０％的神经元分别呈ＣＧＲＰｍＲＮＡ、ＰＰＴＡｍＲＮＡ和ＮＯＳ阳性。ＣＡＲ刺激后，在刺激侧ＴＧ、ＣＧＲＰｍＲＮＡ、ＰＰＴＡｍＲＮＡ和ＮＯＳ阳性神经元的数量均增多，其阳性率分别达到了３８．６％、２２．８％和１４．２％。ＴＧ中约有６．８％和７．３％的神经元同时呈ＮＯＳ和ＣＧＲＰｍＲＮＡ或ＮＯＳ和ＰＰＴＡｍＲＮＡ阳性，ＣＡＲ刺激使得两种双标神经元的数量也明显增多，双标细胞占ＴＧ细胞总数的百分比分别达到了１１．０％和１０．９％，而且刺激后增加的ＮＯＳ阳性神经元主要为双标神经元。上述结果提示ＣＧＲＰｍＲＮＡ、ＰＰＴＡｍＲＮＡ和ＮＯＳ阳性神经元，尤其是共存神经元可能在面口部伤害性信息的传递和调节中起重要作用，伤害性刺激所诱导的ＮＯＳ合成的增加与ＣＧＲＰｍＲＮＡ和ＰＰＴＡｍＲＮＡ表达的增强可能有密切的关系。

The changes of CGRP mRNA,PPTA mRNA and nitric oxide synthase(NOS) expression were examined using NADPH d histochemistry combined with in situ hybridization histochemistry after carrageenan (CAR) induced inflammation.The results showed that about 32 2%,16 2% and 10% trigeminal ganglion(TG) neurons were CGRP mRNA,PPTA mRNA and NOS positive neurons.The numbers of these positive neurons increased significantly after CAR injection.The percentage of these positive neurons reached to 38 6%,22 8% and 14 2%,respectively.About 6 8% and 7 3% of TG neurons were NOS and CGRP mRNA or NOS and PPTA mRNA colocalization neurons.These neurons were small to medium in size.CAR stimulation also resulted in the increase in the number of these colocalization neurons.Collectively,these data suggest that NOS,CGRP mRNA and PPTA mRNA positive neurons,especially their colocalization neurons,might play important roles in oro facial nociceptive processing.