大鼠双后肢高能爆炸伤后远位器官的研究

THE STUDY OF REMOTE ORGANS OF THE RAT AFTER HIGH ENERGY EXPLOSIVE WOUND IN BOTH HIND LEGS

为了探讨大鼠双后肢高能爆炸伤后，远位器官（脑、小肠、膀胱）的形态学改变和这些器官中的生长抑素（ＳＯＭ）和促肾上腺皮质激素释放因子（ＣＲＦ）的变化，用原位杂交组织化学、免疫组织化学、常规光、电镜技术和图像分析等方法作为研究手段，结果显示：１．大鼠双后肢炸伤后，超微结构有明显变化，主要表现为小肠、膀胱中的血管内皮皱缩、胞质内吞饮小泡增多及脑内神经毡水肿和轴突退行性变。２．炸伤后，远位器官中ＳＯＭ及ＳＯＭｍＲＮＡ和ＣＲＦｍＲＮＡ均有一过性的、应激性的变化。３．炸伤后，远位器官中不仅神经内分泌细胞ＳＯＭ和ＣＲＦ表达增强，而且在小肠和膀胱结缔组织中，也出现了高表达的ＳＯＭｍＲＮＡ和ＣＲＦｍＲＮＡ信号细胞，性质待定。本研究提示：高能爆炸伤可致远位器官损伤，ＳＯＭ和ＣＲＦ参与了大鼠炸伤后的创伤应激反应和远达效应的发生。

By using in in situ hybridization, immunochemistry, HE staining, electron microscopy and image analysis, we studied the remote organs (the brain, the small intestine and the urinary bladder) of the rat with high energy explosive wound (HEEW) in both hind legs. The results showed: 1 Obvious pathological ultrastructure changes were observed after the HEEW. Especially, the shrinkage of endothelial cells of blood vessles in the small intestine and the urinary bladder; the neuropil edema and axon degeneration appeared in hypothalamus. 2 The expression of somatostatin (SOM) and its mRNA and corticotropin releasing factor (CRF) mRNA were transiently increased in the brain, the small intestine and the urinary bladder of the rat with HEEW. 3 The SOMmRNA and CRFmRNA were not only expressed in neuroendocrine cells but also in some other cells in small intestine and urinary bladder at 1h after the HEEW. The results suggested that:1 SOM and its mRNA as well as CRFmRNA are involved in the remote effects of HEEW. 2 There are some pathological ultrastructure changes in some remote organs of the rat after the HEEW in both hind legs.