高纯度天然番茄红素中二氯甲烷溶剂脱除工艺的研究

Study on the processing of removing dichloromethane from natural lycopene

以乙酸异丁酯为溶剂,对高纯度天然番茄红素中二氯甲烷的脱除方法进行了初步探究。以二氯甲烷的残留量、天然番茄红素的含量及收率为主要指标,采用单因素变量法,对乙酸异丁酯的使用量、搅拌时间、搅拌温度、乙醇浓度、乙醇使用量、精制搅拌时间等6个因素进行考察。实验结果表明:50倍(v/w)乙酸异丁酯溶解番茄红素,在80℃下搅拌回流3h,再经真空回收溶剂,加入无水乙醇在75℃下保温精制2h,缓慢冷却结晶2h,这一方案对番茄红素中的二氯甲烷具有很好的脱除效果且在保证天然番茄红素高纯度的同时有较好的收率。

The optimal technology to remove dichloromethane from natural lycopene with isobutyl acetate was investigated.With the solvent residues concentrate and content as well as recovery of final product as indexes,factors including amount of isobutyl acetate,stirring time,temperature,ethanol concentration and volume,refining time were analyzed with single factor analysis method.At last the optimal processes to remove dichloromethane from natural lycopene,in which natural lycopene crystal was dissolved in 50 times isobutyl acetate（v/w）at the temperature of 80℃for 3hr,after distillating of the solvent,anhydrous ethanol with volume of 10times（v/w）was added and then refined at 75℃ for 2.0 hr followed crystallized during decreasing the temperature slowly,were obtained.Good effects in the removing harmful solvents and high purity as well as nice recovery could be gained in the processes.