脉冲电磁场刺激对鼠坐骨神经移植影响的实验研究

Effect of Pulsed Electromagnetic Fields Stimulation on the Repair of Peripheral Nerve

目的:研究脉冲电磁场(PEMF)刺激对鼠坐骨神经移植影响。方法:用100只Wistar大白鼠制成坐骨神经移植的模型,随机均分成A组(对照组)和B组(PEMF组),从脊髓、神经及小腿三头肌三个部位同时观察其生物学效应。结果:PEMF刺激具有激活脊髓中枢神经元的活力,加速损伤神经远段Wallerian变性进程,促进雪旺氏细胞(SC)增殖,较快、较早、较多地使神经再生,减慢肌肉(靶器官)失神经后废用等综合效应,最终促使损伤神经的再生和功能的良好恢复。结论:PEMF刺激有利于神经再生。

Aim: To study the effect of pulsed electromagnetic fields(PEMF) stimulation on the repair of rats grafted sciatic nerve. Methods: One hundred Wistar rats were classified randomly into two groups, a control group (group A, n = 50) and a PEMF group(group B, n = 50). The biological effect of the grafted nerve, the relative lumbar spinal cord and one of the ending organs - triceps muscle was studied at the same time. Results: The neuron's vitality in lumber spinal cord had been stimulated in the PEMF- treated rats. The Wallerian degeneration of the nerve distal to the grafted was accelerated after PEMF stimulation. The increased regeneration of the nerve was found throughout 16 weeks recovery period in PEMF group. Application of PEMF had decreased the atrophy of the triceps muscle caused by denervation and promoted its functional recovery. Conclusion: PEMF stimula- tion may enhance the regeneration of the injured peripheral nerve.