金冠×四倍体嘎拉杂种F_1代的倍性分析

Ploidy Analysis of Hybrid F_1 Individuals through Golden Delicious×Tetraploid Gala

【目的】对金冠×四倍体嘎拉杂种F1代的倍性组成进行分析,以便鉴定出三倍体新种质;同时,为今后多倍体育种的亲本选择提供借鉴。【方法】以田间F1代植株茎尖幼嫩叶片为试材,利用流式细胞仪对杂种F1代个体进行了分析。【结果】32株的倍性得到了确认:包括三倍体27株,二倍体4株,单倍体1株,未发现有四倍体植株。三倍体比率高达84.4%,二倍体和单倍体的比率分别为12.5%和3.1%。【结论】金冠×四倍体嘎拉杂交组合是一个优良的创制三倍体新品种/种质的组合,今后可以利用其进行更大规模的杂交育种。

[ Objective] In this study, the objective is to obtain triploid apple germplasm by analyzing the ploidy of hybrid F1 individuals through Golden Delicious × Tetroploid Gala, and provide reference of parents selection. [Method]Flow Cytometry was used to determine the ploidy of young leaves of F1 individuals in field. [ Result] Thirty two individuals were identified, including 4 diploid, 27 triploid and 1 haploid individuals, but there were not tetroploid individuals appearing among Fl individuals. The rate of triploid individuals was up to 84.4 %, the rate of diploid and haploid was 12.5% and 3.1% respectively. [ Conclusion] The combination of＇ Golden Delicious＇ × ＇tetraploid Gala＇ was a good cross combination for creating triploid euhivars/germplasm, which eould be used to breed on a large scale.