鸡肾型传染性支气管炎病毒的分离鉴定与防治

Isolation and Identification of Avian Infectious Bronchitis Nephritis Virus and Its Control

【目的】对新疆疑似肾型传染性支气管炎发病鸡病料进行病毒分离和鉴定。【方法】采用3种不同的治疗方案对发病鸡群进行治疗。【结果】经过处理的病料组织在SPF鸡胚上盲传至第3～4代后，鸡胚出现尿囊绒毛膜增厚，鸡胚卷缩变小、侏儒胚等鸡传染性支气管炎的特征性病变。将收获的病毒液进行电镜观察、病毒中和试验、病毒干扰试验、动物回归试验等证实，分离的3株病毒均属于鸡肾型传染性支气管炎病毒。【结论】电解多维＋肾肿散（中药）＋阿莫西林组合的治疗效果最好，治愈率达到80％～90％。

[ Objective and Method]Three virus strains were isolated from ill chickens with clinical symptom of nephritis. By inoculating the treated tissue fluid to the SPF embryos for 3 - 4 passages, which indicated that the embryos allantoehorion turned thicker and the embryos curled up and dwarfed. [ Result ] The results demonstrated that the isolated three virus strains were diagnosed as infectious bronchitis nephritis by morphological observation with electron microscope, embryo neutralization test, virus interference test and reeursive test in chicken. The ill chickens were treated with three different treatments. [ Conclusion ] The treatment of complex vitamin ＋ kidney - detumescence - powder （ Chinese herbal drug） ＋ amoxicillin is the best and the cure rate was 80 % - 90 %.