产β-内酰胺酶金黄色葡萄球菌的耐药性监测

Monitoring of Antimicrobial Resistance of Staphylococcus Aureus Strains Producing β-lactamase

目的:探讨大理地区产β-内酰胺酶金黄色葡萄球菌的耐药情况。方法:采用VITEK-32型全自动微生物鉴定仪将葡萄球菌属鉴定到种,头孢硝噻吩滤纸片法检测β-内酰胺酶,药物敏感试验采用K-B法。结果:61株金黄色葡萄球菌中产β-内酰胺酶13株,产酶率21.3%,产酶株对万古霉素最敏感,其次为左氧氟沙星,对其他抗生素的耐药率前四位分别是氨苄西林92.4%,青霉素G92.4%,红霉素84.7%,克林霉素69.3%。结论:金黄色葡萄球菌的β内酰胺酶检测应作为常规项目开展,产β-内酰胺酶的金黄色葡萄球菌对青霉素类,大环内酯类抗生素高度耐药,万古霉素无耐药菌株,为首选药物,应在药敏试验指导下合理选用抗生素。

Objective： To investigate the characteristics of antimicrobial resistance of Staphylococcus aureus strains producing β-lactamase which were isolated from clinic in local hospitals. Methods： By using VITEK-32 automated microbiology system for identification of Staphylococcus, β- lactamase in Staphylococcus aureus was detected by Nitrocefin paper. Antimicrobial susceptibility was detected by the disk diffusion method. Results： Of 61 strains of Staphylococcus aurens, 13 strains produce β-1aetamase and the rate of producing enzymes was 21.3% .The strains producing enzymes were most sensitive to Vancomycin and more sensitive to Levofloxaein. The resistant rate of ampieillin and peneillin G were 92.4% and erythromycin was 84.7%; elindamyein was 69.3%. Conclusions： The detection of β- lactamase in Staphylococcus aureus must be a normal item. The strains producing β-lactamase were highly resistant to penicillins and macrolides. Vancomycin must he the first choice in anti-infection caused by Staphylococcus aureus. Antibiotics should be chosen rationally under the guide of the microbial sensitivity test.