摘要
目的:观察转化生长因子β1(TGF-β1)作用下绒癌JEG-3细胞的增殖及其Smad3,7 mRNA的表达变化,进一步探讨TGF-β/Smads信号通路在绒癌发生发展中的作用机制。方法:分别取不同浓度和不同作用时间的TGF-β1处理绒癌JEG-3细胞,采用四甲基氮唑法(MTT)检测细胞的增殖活力,RT-PCR技术检测Smad3,7 mRNA的表达差异。结果:TGF-β1促进了绒癌JEG-3细胞的增殖;随着TGF-β1应用浓度的增高,Smad3 mRNA的表达水平逐渐升高,用一定浓度的TGF-β1作用12h后Smad3 mRNA的表达量随着时间的延长开始逐渐增加;同样条件下Smad7 mRNA的表达差异无统计学意义。结论:TGF-β1可促进绒癌JEG-3细胞的增殖;Smad3mRNA对外源性的TGF-β1有良好的反应性,Smad7 mRNA则表现为无应答。
Objective： To observe the proliferation of JEG -3 choriocarcinoma cells and Smad 3, 7 mRNA expression changes under the action of transforming growth factor β1 （ TGF - β1 ） , explore the mechanism of TGF - β/Smads signal channel in occurrence and development of choriocarcinoma. Methods： The JEG -3 choriocarcinoma cells were treated with different concentrations and times of TGF - β1, the cell proliferation activity was detected by MTF method, the difference of Smad 3, 7 mRNA expression was detected by RT - PCR. Results： TGF- β1 stimulated the proliferation of JEG -3 choriocarcinoma cell; the expression level of Smad 3 mRNA gradually increased with the increase of TGF - β1 concentration; after treated with a certain concentration of TGF - β1 for 12 hours, the expression level of Smad 3 mRNA gradually increased with time passing ; under the same condition, there was no significant difference in expression level of Smad 7. Conclusion： TGF - β1 can stimulates the proliferation of JEG -3 choriocarcinoma cell; the reaction of Smad 3 mRNA to exogenous TGF - β1 is good, while Smad 7 mRNA has no response.
出处
《中国妇幼保健》
CAS
北大核心
2010年第12期1685-1689,共5页
Maternal and Child Health Care of China
基金
河北省卫生厅课题资助项目〔05015〕
河北省科学技术研究与发展计划项目〔07276101D-35〕
河北省教育厅2008年自然科学研究计划项目〔2008303〕
