水稻雄性不育系及雄性不育突变体筛选研究进展

ADVANCES IN SCREENING OF CMS LINE AND MALE STERILE MUTANT IN RICE(ORYZA SATIVA)

为了获得新细胞质源的水稻雄性不育系及雄性不育突变体，以解决目前杂交水稻生产上所面临的不育系来源单一的问题，人们采用了常规育种、诱变育种、组织培养和离体诱变等方法，其中离体筛选尤显其优势性，但也有不少问题有待解决，如能通过离体培养定向地筛选出雄性不育系及雄性不育突变体，无疑将大大提高工作效率。此外，对影响离体筛选雄性不育突变体的因素进行了探讨。

In order to obtain new typed cytoplasmic male sterile(CMS)line and male sterile mutant of rice,many methods,such as conventional breeding,induced breeding,tissue culture and in vitro induced mutation,are used to solve the problem of single source of cytoplasm in hybrid rice production,among which,in vitro screening shows conspicuous preponderance,though some questions still exist.It is no doubt that the male sterile frequency will increase considerably if male sterile mutants can be obtained directively through in vitro culture.In addition,factors affecting the in vitro screening of male sterile mutants are also discussed in this paper.