戊二醛浓度对免疫电镜胶体金标记密度影响的定量分析

QUANTITATIVE ANALYSIS OF THE EFFECT OF GLUTARALDEHYDE CONCENTRATION ON THE LABELLING DENSITY IMMUNOCHEMICAL LOCALIZATION OF PROTEIN A COL LOIDAL GOLD PARTICLES

经４％多聚甲醛＋０或０５％戊二醛固定（１小时）大豆子叶细胞，大部分细胞器和蛋白体发生较大程度的变形，甚至解体。随着固定剂中戊二醛浓度升高至１５、２５％，细胞超微结构保存效果提高，蛋白体内反映７Ｓ蛋白质存在的胶体金颗粒密度分别为１９７和１１４个／μｍ２。

Most organelles and protein bodies swelled to a large extent, or were ever destroyed in the soybean cotyledon cells fixed with fixatives of 4% formaldehyde+0,0.5% glutaraldehyde. But the glutaraldehyde fixation with 1.5, 2.5% formaldehyde were used as a compromise between structural and antigenic preservation, resulting in the labeling density of 19.7 and 11.4/μm 2 of colloidal gold particles on the surface of protein matrix in the protein bodies, respectively.