DIG系统在Northern杂交中的应用

THE APPLICATION OF DIG SYSTEM TO NORTHERN BLOT

非放射性标记已越来越广泛地被应用于分子生物学的研究。本文介绍本实验利用ＤＩＧ高效ＤＮＡ标记系统进行Ｎｏｒｔｈｅｒｎｂｌｏｔ的经验，供同行参考。

Non radioactive labeling technique is now widely accepted as a routine procedure in molecular biology researches. This report introduced our protocol of using DIG labeled DNA probes in Northern blot, which is sensitive enough to detect the light regulated expression of spinach rbcL gene.