基于油镜的全自动显微图像扫描采集系统研发及在疟疾诊断中的应用

Development of oil lens-based fully automatic microscopy graphics scanning system and its preliminary application in diagnosis of malaria

目的研发基于油镜的全自动显微图像扫描采集系统,用以替代传统的人工镜检读片,提高疟疾镜检效率和疟原虫检出率。方法基于油镜的全自动显微图像扫描采集系统由光学显微镜、数码摄像机/模拟摄像机、控制软件和普通计算机组成。采用盲法,用基于油镜的全自动显微图像扫描采集系统和传统镜下读片,各检读10张血片样本,每张血片读100个视野,并将读得的阳性血片计数。记录每个镜检员检读每张血片样本的时间,并计算读片准确率、读片速度、阳性血片和阴性血片的符合率。结果该系统目前实现的基本功能为自动位移和对焦,自动扫描和储存,自动回位和复位功能,标注和自动计数,报告单打印等。系统在疟疾诊断中初步应用效果:该系统较传统读片速度提高30.58%,读片的总符合率提高13.33个百分点。结论该系统在疟疾血片读片速度和准确度方面均优于传统镜下读片,且该系统操作简单、价格便宜、易于推广。

Objective To establish an automatic microscope scanning system based on the oil-lens for replacing the traditional manual microscopy reading of blood films to improve the efficiency and the detection rate of Plasmodium.Methods The system consisted of an optical microscope,digital camera,control software and general computer-based component.The system and professional persons read the blood films with single-blind method,everyone read 10 blood samples（100 fields of vision per blood film）,and the time and results of reading were recorded.Results The system had the function of automatic displacement and focus,automatic scanning and storage,automatic back-bit and reset,annotation,and automatic counting,reporting and printing.The system can increased the speed of reading films by 30.58%,and improved the accuracy by 13.33%.Conclusion The automatic microscope scanning system can improve the speed and accuracy of reading films and the operation is simple.