摘要
利用AFLP技术对44份番茄材料进行遗传多样性和亲缘关系分析。从64对引物组合中筛选出15对用于扩增,共获得601条带,其中多态性带364条,多态性为60.5%,这表明供试材料在DNA水平上酶切位点的分布存在广泛的变异。利用UPGMA法将44份番茄材料分成5个复合组和1个独立组,从相似性系数分析了各材料之间的亲缘关系。
The genetic diversity and relationship of 44 tomato accessions were analyzed by AFLP （amplified fragment length polymorphism）. Fifteen primer combinations selected from 64 primer combinations produced a total of 601 fragments, 364 of which were polymorphic with a polymorphism percentage of 60.5%. This results showed that there were abundant diversities in enzyme digestion sites among different tomato varieties. These tomato germplasm resources were clustered into 5 multiple-member groups and 1 one-member group by UPGMA （unweighted pair group method with arithmetic average） based on Nei＇s genetic distances. The genetic relationship of 44 tomato accessions was analyzed using the different coefficient.
出处
《浙江农业学报》
CSCD
北大核心
2010年第2期156-160,共5页
Acta Agriculturae Zhejiangensis
基金
浙江省科技计划项目(2007D70009)
农业科技成果转化资金项目(2007GB2C200129)
浙江省科技厅重大科技专项(优先主题)农业项目(2008C120022)
