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芥蓝Brassica albograbra花青素合成酶基因BaANS的克隆与序列分析 被引量：6

Isolation and sequence analysis of anthocyanidin synthase gene BaANS from Brassica albograbra
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摘要 根据NCBI数据库中的已知序列设计简并引物,分别从芥蓝(Brassica albograbra)叶片基因组DNA和cDNA中克隆到了花青素合成酶基因。基因命名为BaANS,该基因全长1369bp,具一个长度为292bp的内含子,编码区长度为1077bp,编码358个氨基酸,序列已提交到NCBI,登录号为GU170203。序列比对结果表明,BaANS与甘蓝、拟南芥、紫罗兰、白菜和芥菜等的ANS有较高的相似性。 Degenerate primer pairs were designed according to known anthocyanidin synthase gene sequences in NCBI database. The gene, designated BaANS, was isolated from leaf genomic DNA and cDNA of Chinese kale （Brassica albograbra）, respectively. Genomic DNA of BaANS gene is 1 369 bp in length with one intro of 292 bp. Complete coding sequence of BaANS is 1 077 bp in length encoding 358 amino acids. The sequence has been submitted to NCBI with accession number of GU170203. Sequence comparison with Matthiola incana, B. oleracea var. capitata, B. juncea, B. rapa ssp. campestris and Arabidopsis thaliana showed that they shared high homology.
作者 赵蓉蓉 蒋明 贺蔡明 朱雅琴 周敏
机构地区 台州学院生命科学学院
出处 《浙江农业学报》 CSCD 北大核心 2010年第2期161-166,共6页 Acta Agriculturae Zhejiangensis
基金 浙江省自然科学基金项目(Y3080081) 台州市科技计划项目(08XH02)
关键词 芥蓝 花青素合成酶 序列分析 Chinese kale （ Brassica albograbra） anthocyanidin synthase sequence analysis
分类号 S635 [农业科学—蔬菜学] Q78 [生物学—分子生物学]
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参考文献21

  • 1Schaefer HM,Wilkinson DM.Red leaves,insects and coevolution:a red herring?[J].Trends in Ecology & Evolution,2004,19:616-618.
  • 2Harborne JB,Williams CA.Advances in flavonoid research since 1992[J].Phytochemistry,2000,55(6):481-504.
  • 3Rice-Evans CA,Miller NJ,Paganga G.Antioxidant properties of phenolic compounds[J].Trends in Plant Science,1997,2:152-158.
  • 4张宁,胡宗利,陈绪清,侯晓姝,李勇,陈国平.植物花青素代谢途径分析及调控模型建立[J].中国生物工程杂志,2008,28(1):97-105. 被引量：67
  • 5Tsuda T,Horio F,Uchida K,et al.Dietary cyanidin 3-O-beta-D-glucoside-rich purple corn color prevents obesity and ameliorates hyperglycemia in mice[J].Journal of Nutrition,2003,133(7):2125-2130.
  • 6Shimada S,Inoue YT,Sakuta M.Anthocyanidin synthase in non-anthocyanin-producing Caryophyllales species[J].Plant Journal,2005,44(6):950-959.
  • 7Gong Z,Yamazaki M,Sugiyama M,et al.Cloning and molecular analysis of structural genes involved in anthocyanin biosynthesis and expressed in a forma-specific manner in Perilla frutescens[J].Plant Molecular Biology,1997,35(6):915-927.
  • 8Szankowski I,Flachowsky H,Li H,et al.Shift in polyphenol profile and sublethal phenotype caused by silencing of anthocyanidin synthase in apple(Malus sp.)[J].Planta,2009,229(3):681-692.
  • 9Xu F,Cheng H,Cai R,et al.Molecular cloning and function analysis of an anthocyanidin synthase gene from ginkgo biloba,and its expression in abiotic stress responses[J].Molecules and Cells,2008,26(6):536-547.
  • 10Jaakola L,M(a)(a)tt(a) K,Pirttil(a) AM,et al.Expression of genes involved in anthocyanin biosynthesis in relation to anthocyanin,proanthocyanidin,and flavonol levels during bilberry fruit development[J].Plant Physiology,2002,130(2):729-739.

二级参考文献93

  • 1黄科,叶纨芝,余小林,向珣,卢钢,曹家树.农杆菌介导的芥蓝遗传转化体系的建立[J].细胞生物学杂志,2007,29(1):147-152. 被引量：7
  • 2Ito M, Ichinose Y, Kato H, et al. Molecular evolution and func- tional relevance of the chalcone synthase genes of pea[J]. Mol Gen Genet, 1997, 255(1): 28-37.
  • 3Holton TA, Brugliera F, Lester DR, et al. Cloning and expression of cytochrome 17450 genes controlling flower color [ J ]. Nature, 1993, 366: 276-279.
  • 4Winkel-Shirley B. Biosynthesis of flavonoids and effects of stress [J]. Curr Opin Plant Biol, 2002, 5: 218-223.
  • 5Murray MG, Thompson WF. Rapid isolation of high molecular weight plant DNA [J]. Nucleic Acids Res, 1980, 8: 4321- 4325.
  • 6Reimold U, Kruger M, Krenzaler F, et al. Coding and 3' non-coding nucleotide sequence of chalcone synthase mRNA and assignment of amino acid sequence of the enzyme [ J ]. EMBO J, 1983, 2:1801 - 1805.
  • 7Jez JM, Noel JP. Mechanism of chalcone synthase: pKa of the catalytic cysteine and the role of the conserved histidine in a plant polyketide synthase [ J ]. J Biol Chem, 2000, 275: 39640- 39646.
  • 8Yamazaki Y, Suh D, Sitthithawom W, et al. Diverse chalcone synthase superfamily enzymes from the most primitive vascular plant Psilotum nudum [J]. Planta, 2001, 214:75 - 84.
  • 9Harashima S, Takano H, Ono K, et al. Chalcone synthase-like gene in the liverwort Marchantia paleacea var. diptera [ J ]. Plant Cell Rep, 2004, 23:167 - 173.
  • 10Jiang C, Schommer CK, Kim SY, et al. Cloning and characterization of chalcone synthase from the moss, Physcomitrella patens [J]. Phytochemistry, 2006, 67(23): 2531 -2540.

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浙江农业学报
2010年 第2期

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