豚鼠气单胞菌超声灭活PEG结合疫苗的制备及免疫效果

Study on preparation and immune efftect of PEG conjugate vaccine of ultrasonic inactived Aeromonas hydrophila

以超声波瞬间高温、高压及剪切力作为灭活方式,用聚乙二醇(PEG)作为超声介质及疫苗载体,将豚鼠气单胞菌制成PEG结合疫苗。该疫苗与传统疫苗分别经腹腔注射免疫接种锦鲤成鱼,经检测血细胞吞噬百分率、吞噬指数、抗菌活力、抗体效价及免疫保护力等指标,评价疫苗效果。结果表明PEG6000作为结合剂制备的疫苗其各指标和绝对值全部高于其他试验组。PEG6000疫苗免疫保护力相比也最高,达到80%,高于传统灭活疫苗26.7个百分点,说明PEG6000作为结合剂制备的疫苗效果最好。

PEG conjugate vaccine of Aeromonas hydrophila was made by the methods of transient high-temperature, high press and shearing force of ultrasonic, using PEG as ultrasonic medium and vaccine vector. The PEG conjugate vaccine and the traditional vaccine were injected Cryprinuscarpiod intraperitioneally respectively. The percentage of phagocytosis, the index of phagocytosis, antibacterial activity, antibody titer and immune protective efficiency were investigated. The results showed that all the testing indexes of PEG conjugate vaccine were significantly higher than other vaccines. The immune protective efficiency was 80% for PEG conjugate vaccine, which was the highest and 26. 7% higher than that of traditional vaccine, indicating that PEG6000 was a good binder of vaccine preparation for Cryprinuscarpiod against Aeromonas hydrophila.