柃木的组织培养与快速繁殖技术

Tissue culture and rapid propagation of Eurya japonica Thunb

为建立柃木的茎尖组培快繁体系,研究了0.2%HgCl2不同灭菌时间、不同6-BA浓度对柃木芽诱导和增殖的影响;以及不同的IBA浓度、糖浓度和生根法,对柃木根诱导和生根的影响。结果表明:外植体用0.2%HgCl2表面灭菌25min,并以WPM为基本培养基,芽诱导、增殖时的6-BA浓度为0.5mg/L,NAA浓度为0.05mg/L,GA浓度为0.2mg/L,蔗糖为20g/L;根诱导时IBA浓度为0.5mg/L和糖浓度为30g/L以及两步生根法等,是柃木组培和快速繁殖的适宜条件。应用此方法的柃木嫩枝的芽诱导萌发率可达100%、芽增殖系数为2.56～4.58/次、生根率为98%、组培苗成活率为95%以上,并已应用于生产。

The cut fresh branches of Eurya japonica in Zhejiang are processed as a kind of traditional forest products to be exported to Japan. In order to establish a rapid propagation system on shoot tip culture of E. japonica, the effects of different sterilization time of HgCl2 on polluted rate of explants, the effects of different 6-BA concentrations on shoot subculture and multiplication culture, the effects of different IBA concentrations, sucrose concentrations and rooting methods on rooting rate were studied. The results showed that the appropriate conditions for tissue culture and rapid propagation of E. japonica were as follows ： 25 min shoot surface-sterilization with 0. 2% HgCl2 , WPM basal medium with 0. 5 mg·L^-1 6-BA, 0. 05 mg·L^-1 NAA, 0. 2 mg·L^-1GA, 20 g. L^-1 sucrose in proliferation culture, 0. 5 mg·L^-1 IBA, 30 g· L^-1 sucrose and two-step rooting method in rooting culture. Under this condition, bud-induction rate, multiplication coefficient, rooting rate and transplant survival rate of tissue culture seedlings could reach 100% , 2. 56 -4. 85 per time, 98% and 〉95% respectively.