山葵中异硫氰酸酯的提取及其对流感病毒的作用

Extraction of Lsothiocyanate in Wasabi Japonica Matsum and Its Effects to Influenza Virus

为了开发山葵的药用价值,采用萃取和蒸溜方法,提取山葵中有效成分异硫氰酸酯,把提取的异硫氰酸酯用血凝试验法和动物试验法对流感病毒的作用进行了试验,以弄清楚山葵中异硫氰酸酯的含量及其作用.研究结果表明,山葵中异硫氰酸酯含量为:须根中0.53mol·mL-1,根茎中0.80mol·mL-1,叶柄中0.27mol·mL-1.山葵中异硫氰酸酯对流感病毒有明显的杀效果,异硫氰酸酯和病毒的比例为0.15mL+0.05mL处理病毒90min,使病毒完全失去活性,不会再引起疾病.这为山葵中异硫氰酸酯的提取及其药用价值提供了科学依据.

With a view to develop medicinal value of Wasabi Japonica Matsum,isothiocyanate,the active ingredient,is extracted by extraction and distillation methods,the extracted isothiocyanate have then been tested on effects against influenza virus by resorting to hemagglutination test and animal experiment methods in getting the content and effects of isothiocyanate in Wasabi Japonica Matsum. The results show that the content of isothiocyanate in Wasabi Japonica Matsum were 0.53mol·mL^-1 in fibrous root,0.80 mol·mL^-1 in rhizome and 0.27 mol·mL^-1 in petiole. Moreover isothiocyanate in Wasabi Japonica Matsum has remarkable effects in killing influenza virus. When the proportion of isothiocyanate and virus is 0.15 mL ＋0.05 mL,it enables virus to lose activity completely after being treated for 90 min,Diseases could not be caused. This study provides scientific basis for isothiocyanate extraction in Wasabi Japonica Matsum and its medicinal value.