摘要
Based on the interaction mechanism between tip and sample in the contact mode of a scanning probe acoustic microscope （SPAM）, an active mass of the sample is introduced in the mass-spring model. The tip motion and frequency response of the sample vibration mode in the SPAM are calculated by the Lagrange equation with dissipation function. For the silicon tip and glass assemblage in the SPAM the frequency response is simulated and it is in agreement with the experimental result. The living myoblast cells on the glass slide are imaged at resonance frequencies of the SPAM system, which are 20 kHz, 30 kHz and 120 kHz. It is shown that good contrast of SPAM images could be obtained when the system is operated at the resonance frequencies of the system in high and low-frequency regions.
Based on the interaction mechanism between tip and sample in the contact mode of a scanning probe acoustic microscope （SPAM）, an active mass of the sample is introduced in the mass-spring model. The tip motion and frequency response of the sample vibration mode in the SPAM are calculated by the Lagrange equation with dissipation function. For the silicon tip and glass assemblage in the SPAM the frequency response is simulated and it is in agreement with the experimental result. The living myoblast cells on the glass slide are imaged at resonance frequencies of the SPAM system, which are 20 kHz, 30 kHz and 120 kHz. It is shown that good contrast of SPAM images could be obtained when the system is operated at the resonance frequencies of the system in high and low-frequency regions.
基金
Supported by the National Natural Science Foundation of China under Grant Nos 10774113 and 10834009, and the Doctoral Fund of Ministry of Education of China under Grant No 20070247042.
