安徽猪源非伤寒沙门菌耐药及多重耐药性的监测研究

Investigation on the drug resistance and multi-drug resistance of nontyphoidal Salmonella isolates from pigs in Anhui

目的了解安徽地区猪源非伤寒沙门菌分离株的多重耐药状况及耐药程度。方法应用标准K-B纸片法对100株沙门菌进行耐药检测,并分析其多重耐药性。结果分属B、E1、E4和F四个血清群的100株非伤寒沙门菌总耐药率为72%,其中B群耐药率为82.35%,E1群为44.44%,E4群为84.62%,F群为100%;72株耐药菌株中有52株为耐3种以上抗生素的多重耐药,其中B群多重耐药率为64.29%,E1群为75%,E4群为45.45%,F群为100%;多重耐药谱是强力霉素—四环素—卡那霉素—氯霉素。结论安徽地区猪源非伤寒沙门菌分离株的多重耐药程度严重,该地区养猪业在使用抗生素方面存在严重问题,应加强对抗生素合理使用的管理以及食品卫生的监督工作。

Objective To understand the drug susceptivity of nontyphoidal Salmonella isolates from pigs in Anhui and evaluate the spectrum of their multi-drug resistance. Method The sensitivity of 100 nontyphoidal Salmonella isolates to 14 antibiotics were determined by K-B method. Result Among the 100 nontyphoidal Salmonella isolates,the overall resistance rate to 14 antibiotics was 72%,in which the resistance rates to 14 antibiotics of serogroup B,serogroup E1,serogroup E4 and serogroup F were 82.35%,44.44%,84.62% and 100% respectively. Fifty-two out of the seventy-two nontyphoidal Salmonella strains exhibited resistance to at least three antimicrobials. The multi-drug resistance rates of serogroup B,serogroup E1,serogroup E4 and serogroup F were 64.29%,75%,45.45% and 100% respectively. The highest frequency of resistance was the frequency of resistance to multiple antimicrobials,including Doxycycline ,Tetracycline,Chloromycetin and Kanamycin. Conclusion The multi-drug resistance is very common among the nontyphoidal Salmonella isolates from pigs in Anhui. The unreasonable use of antimicrobials is severe in domestic livestock. It is necessary to enhance the management of reasonable antimicrobial agents use and the supervision of food hygiene.