COX-2、Ki-67和结核菌L型感染在前列腺癌中的表达

Expression and clinical significance of COX-2,Ki-67 and tuberculosis infection L-type in prostate carcinoma

目的探讨环氧合酶-2(COX-2)和Ki-67在前列腺癌中的表达以及结核菌L型感染率及临床意义。方法应用免疫组化、原位杂交和抗酸染色等方法检测了65例前列腺癌(carcinoma of prostate,PCa)和30例良性前列腺增生(benign prostatic hyperplasia,BPH)中的COX-2、Ki-67蛋白及mRNA的表达,以及结核菌L型的检出率;并对前列腺肿瘤主要临床资料和病理分级参数进行比较,用χ2检验进行统计学处理。结果COX-2、Ki-67蛋白及mRNA阳性表达和结核菌L型检出率,前列腺癌明显高于前列腺增生(P<0.001～0.05)。COX-2、Ki-67蛋白及mRNA阳性表达和结核菌L型检出率与前列腺癌的临床分期、病理分级有明显差异(P<0.01～0.05)。淋巴结转移组中COX-2、Ki-67蛋白及mRNA的阳性表达率明显高于非转移组(P<0.01)。结核菌L型检出率淋巴结转移组明显高于非转移组(P<0.05)。结论COX-2、Ki-67蛋白及mRNA在前列腺肿瘤中不同程度异常表达以及结核菌L型检出率与肿瘤的临床分期、病理分级和转移呈正相关,提示2种基因均可作为判断前列腺癌生物学行为及患者预后参考指标。结核菌L型感染极有可能导致基因的变异或过表达,成为诱发肿瘤因素之一,它们可能有协同致瘤作用。

Objective To explore Cox-2 and Ki-67 in prostate carcinoma as well as L-type Mycobacterium tuberculosis infection and its clinical significance.Method Immunohistochemistry,in situ hybridization and other detection methods such as acid-fast stain were applied to 65 cases of prostate cancer （carcinoma of prostate,PCa） and 30 cases of benign prostatic hyperplasia （benign prostatic hyperplasia,BPH） for the expressions of Cox-2,Ki-67 protein and mRNA,as well as the detection rate of TB L-type. The main clinical data and pathological classification parameters of prostate cancer were analyzed statistically with χ^2 test. Result The expressions of Cox-2 and Ki-67 proteins and mRNA as well as the L-TB detection rate in prostate cancer was significantly higher than those in benign prostatic hyperplasia （P〈0.001-0.05）. The expressions of Cox-2 and Ki-67 proteins and mRNA as well as L-TB detection rates in prostate cancer at different clinical stages,histological grades were significantly different （P〈0.01-0.05）. The Lymph node metastasis group showed significantly higher positive expression rate of cox-2 and ki-67 proteins and mRNA than the non-metastasis group did （P〈0.01）. L-TB detection rate of lymph node metastasis group was significantly higher than that of non-metastasis group （P〈0.05）.Conclusion Cox-2 and Ki-67 proteins and the abnormal expression of mRNA in prostate tumors as well as the L-TB detection rate are positively correlated with clinical tumor stage,grade and metastasis,suggesting that the two genes can be used as the reference indicators of biological behavior and prognosis of patients with prostate cancer. L-TB infection very likely leads to gene mutation or overexpression,and become one of the inducing factors of tumors; they may have synergistic tumorigenic effect.