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抗癫癎药物诱导对血脑屏障上P-糖蛋白功能与表达的影响 被引量：2

Antiepileptic drugs up-regulated activity and expression of P-glycoprotein in blood-brain barrier of rats
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摘要 目的研究长期使用抗癫疒间药物(AEDs)对大鼠脑中P-糖蛋白(P-gp)功能活性和表达水平的影响。方法选择苯巴比妥、苯妥英钠和卡马西平三个典型AEDs,在大鼠体内连续给药21天,然后观察脑组织中皮质和海马P-gp的改变。结果底物罗丹明123转运实验表明P-gp功能增强,进一步通过Western Blot分析表明P-gp表达增强,并用免疫组化染色显示其细胞定位在血脑屏障上。结论长期AEDs诱导可以同时导致大鼠血脑屏障上P-gp功能活性和表达水平的升高。 Objective To investigate whether chronic exposure of antiepileptic drugs（AEDs） increased P-glycoprotein（P-gp） function and expression in brain of rats.Methods Three drugs phenobarbital（PB）,phenytion（PHT） and carbamazepine（CBZ） were orally given to rats for successive 21 days,P-gp activity and levels in brain was assessed.Results Tissue-to-plasma concentration ratios of Rho123 were significantly decreased in cerebral cortex and hippocampus.Immunohistochemistry result showed that up-regulation of the P-gp mainly occurred in capillary endothelial vessels.Western blot result suggested that the protein level of P-gp in cortex and hippocampus of rats exposed to drugs was significantly higher than that of control rats.Conclusion Chronic exposure of AEDs may increase P-gp function and level in brain of rats.
作者 王琳琳 刘晓东 王靖元
机构地区 中国药科大学药学院 赣榆县卫生局
出处 《徐州医学院学报》 CAS 2010年第4期230-233,共4页 Acta Academiae Medicinae Xuzhou
关键词 P-糖蛋白 血脑屏障 抗癫间疒药物 诱导 P-glycoprotein Blood-brain barrier Antiepileptic drugs Induction
分类号 R971.6 [医药卫生—药品] R96 [医药卫生—药理学]
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徐州医学院学报
2010年 第4期

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