门冬氨酸与2,3-二氯-5,6-二氰-1,4-苯醌荷移反应的研究

Spectrophotometric study of aspartic acid with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone based on charge transfer reaction

目的：用分光光度法研究门冬氨酸与2,3-二氯-5,6-二氰-1,4-苯醌（DDQ）的荷移反应。方法：门冬氨酸与DDQ于pH9.5的三酸缓冲溶液中,60℃水浴中加热15 min,可生成稳定的荷移络合物。结果：荷移络合物的组成比为1：1,λmax=342 nm,表观摩尔吸光系数ε=3.42×103L/mol.cm,在10~30μg/ml的范围内符合比尔定律,回收率为97.1%~101.7%,相对标准偏差在2.9%以内。结论：应用本方法测定门冬氨酸样品的含量,结果与文献方法一致。

Objective：To develop a spectrophotometric method for determination of aspartic acid.Methods：It was based on a charge transfer reaction of the studied drug with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone（DDQ） in B-R（pH9.5） buffer solution at 60℃ of water-bath for 15 min.Results：The composition of the charge transfer complex was 1：1.The apparent molor absorptivity was determined to be 3.42×103 L/mol·cm at 342 nm,and Beer′s law was obeyed in the range of 10～30 μg/ml.The relative standard deviation was less than 2.9% and the recovery was in the range of 97.1%～101.7%.Conclusion：The developed method could be applied successfully for the determination of aspartic acid in its pharmaceutical dosage forms with a good precision and accuracy compared to official methods.