液相色谱法测定辣椒制品中苏丹红

HPLC analysis of Sudan dyes in chili spices

目的：高效液相色谱法同时测定食品中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ。方法：样品经萃取、浓缩后,用C18柱分离,二极管阵列检测器定性、定量。结果：该方法苏丹红的线性范围均为0.125~20.0μg/ml,检出限为4.4~9.1μg/kg,辣椒粉的加标回收率为98.1%~105%。结论：该方法较灵敏,准确,可用于食品中苏丹红的常规检测。

Objective：Simultaneous determination of Sudan dyes I,Ⅱ,Ⅲ,Ⅳ was performed by employing high performance liquid chromatography.Methods：Samples were extracted,condensed,and then separated with C18 column,qualitatively and quantitatively analyzed by high performance liquid chromatography-diode array detector.Results：Linear range of the method was 0.125~20.0 μg/ml,and the minimum detective limit was 4.4~9.1 μg/kg,spike recovery in pepper powder was 98.1%~105%.Conclusion：This method is quite sensitive,accurate and can be used for routine determination of Sudan dyes in food.