AMS-8-NH_2的合成及其对ODN的负载释放

Synthesis of AMS-8-NH_2 and Its Loading-release of Oligodeoxyneucleotide

以阴离子表面活性剂肉豆蔻酰基谷氨酸钠(C14GluA-Na)为模板剂,3-氨丙基三甲氧基硅烷(APS)为辅助结构导向剂,合成了介孔材料AMS-8-NH2,并结合XRD分析、N2吸附/脱附技术及29Si魔角旋转核磁共振(MAS NMR)等技术手段对纳米孔洞材料AMS-8-NH2进行了表征.结果表明,AMS-8-NH2是高度有序的纳米孔洞材料,其孔径和比表面积分别为3.2nm和706m2/g;有机胺基团通过化学键连接在材料内表面,这些基团的覆盖度达到25%.以AMS-8-NH2为载体,寡核苷酸(ODN)为模型生物分子研究在不同条件下AMS-8-NH2对ODN的吸附和释放性能,实验结果表明,当ODN浓度一定时,溶液的pH值越低,对ODN的吸附量越大,pH=4.7时对ODN的最大负载量可达214mg/g;在释放过程中,材料对ODN具有较高的缓释能力,随环境pH值增加,释放量增加.

Mesoporous silicas of AMS-8-NH2 have been synthesized with the anionic surfactant N-myristoyl-Lglutamic acid sodium（C 14 GluA-Na） and 3-amino propyltrimethoxy silane（APS） as co-structure-directing agents（CSDA） .The materials were characterized by powder X-ray diffraction（XRD） ,N 2 adsorption/desorption analysis and 29 Si magic angle spinning nuclear magnetic resonance（MAS NMR） .The results exhibit that the structure of AMS-8-NH2 has a long range order of three-dimensional cage-like connecting aperture with about 3.2 nm pore diameter and 706 m 2 /g special surface area respectively nano-hole material.Organic amine groups have anchored chemically to the surface of material through chemical bonding,whose coverage has reached 25%.The resulting AMS-8-NH2 materials were used as vehicles and herring sperm oligodeoxyneucleotide（ODN） as a model biological molecule for discussing the loading and release character of ODN on AMS8-NH2.The loading experiment shows that when the solution concentration is fixed,the higher solution acidity,the higher loading capability and the maximum amount can reach 214 mg/g at pH=4.7.Among the release process,high sustained release effect of ODN on materials is displayed,which is controlled by environmental pH value.The greater the pH value,the higher the release amount.