小蜡树的酚苷及苯乙醇苷类成分

Phenolic and phenylethanoidal glycosides from branch of Fraxinus sieboldiana

目的：系统研究小蜡树Fraxinuxsieboldiana枝乙醇提取物的化学成分。方法：运用正相和反相硅胶、大孔吸附树脂、凝胶、反相高效液相等色谱方法进行分离;应用包括一维和二维NMR等波谱方法鉴定化合物的结构。结果：从小蜡树枝的乙醇提取物水相部位分离得到4个酚苷和12个苯乙醇苷类成分,分别鉴定为：2,6-二甲氧基-对苯二酚-4-O-β-D-吡喃葡萄糖苷（1）,2,6-二甲氧基-对苯二酚-1-O-β-D-吡喃葡萄糖苷（2）,4-羟基-3-甲氧基苯基β-D-吡喃葡萄糖苷（3）,4-羟基-3-甲氧基苯基β-D-吡喃木糖基（1→6）-O-β-D-吡喃葡萄糖苷（4）,osmanthuside H（5）,2-（4-羟基苯基）乙基β-D-吡喃葡萄糖苷（6）,2-（3,4-二羟基苯基）乙基β-D-吡喃葡萄糖苷（7）,2-羟基-4-（2-羟基乙基）-苯基β-D-吡喃葡萄糖苷（8）,4-（2-羟基乙基）-2-甲氧基苯基β-D-吡喃葡萄糖苷（9）,calceolarioside B（10）,calceolarioside A（11）,ferruginoside A（12）,isolugrandoside（13）,类叶升麻苷（14）,chiritotoside C（15）和plantasisoside（16）。结论：化合物1-4,9,12,13和16为首次从该属植物中分离得到。

Objective：To investigate chemical constituents from an ethanolic extract of the branch of Fraxinus sieboldiana（Oleaceaue）.Method：The constituents were isolated and purified by a combination of various chromatographic techniques including silica gel,macroporous adsorbent resin,Sephadex LH-20,and preparative HPLC.Structures of the isolates were elucidated by spectroscopic methods including 1D and 2D NMR and MS techniques.Result：Four phenolic and twelve phenylethanoidal glycosides were obtained and their structures were identified as 2,6-dimethoxy-p-hydroquinone-4-O-β-D-glucopyranoside（1）,2,6-dimethoxy-p-hydroquinone-1-O-β-D-glucopyranoside（2）,4-hydroxy-3-methoxyphenyl β-D-glucopyranoside（3）,4-hydroxy-3-methoxyphenyl β-D-xylopyranosyl（1→6）-O-β-D-glucopyranoside（4）,osmanthuside H（5）,2-（4-hydroxyphenyl） ethyl β-D-glucopyranoside（6）,2-（3,4-dihydroxyphenyl） ethyl β-D-glucopyranoside（7）,2-hydroxy-4-（2-hydroxyethyl）-phenyl β-D-glucopyranoside（8）,4-（2-hydroxyethyl）-2-methoxyphenyl β-D-glucopyranoside（9）,calceolarioside B（10）,calceolarioside A（11）,ferruginoside A（12）,isolugrandoside（13）,acteoside（14）,chiritotoside C（15）,and plantasisoside（16）.Conclusion： Compounds 1-4,9,12,13 and 16 were obtained from the genus Fraxinus for the first time.